Single-copy nuclear gene primers for Streptanthus and other Brassicaceae from genomic scans, published data, and ESTs1

UNLABELLED PREMISE OF THE STUDY We report 11 primer sets for nine single-copy nuclear genes in Streptanthus and other Thelypodieae (Brassicaceae) and their utility at tribal-level and species-level phylogenetics in this poorly resolved group. • METHODS AND RESULTS We selected regions based on a cross-referenced matrix of previous studies and public Brassica expressed sequence tags. To design primers, we used alignments of low-depth-coverage Illumina sequencing of genomic DNA for two species of Brassica mapped onto Arabidopsis thaliana. We report several primer combinations for five regions that consistently amplified a single band and yielded high-quality sequences for at least 70% of the species assayed, and for four additional regions whose utility might be clade specific. • CONCLUSIONS Our primers will be useful in improving resolution at shallow depths across the Thelypodieae, and likely in other Brassicaceae.